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Related Experiment Videos

Endogenous digitalis-like activity in mammalian brain.

M C Fishman

    Proceedings of the National Academy of Sciences of the United States of America
    |September 1, 1979
    PubMed
    Summary

    Researchers identified a brain substance that mimics digitalis glycosides by blocking [3H]ouabain binding to Na+,K+-ATPase and inhibiting 86Rb+ uptake in human red blood cells.

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    Area of Science:

    • Biochemistry
    • Neuroscience
    • Cell Physiology

    Background:

    • The brain contains endogenous substances that may influence cellular ion transport.
    • Digitalis glycosides are known cardiac drugs that interact with Na+,K+-ATPase.

    Purpose of the Study:

    • To isolate and characterize a brain-derived substance with digitalis-like activity.
    • To investigate the substance's effect on Na+,K+-ATPase and ion transport.

    Main Methods:

    • Preparation and fractionation of brain tissue using Sephadex G-10.
    • Assay of [3H]ouabain binding to Na+,K+-ATPase.
    • Measurement of 86Rb+ uptake in human erythrocytes.

    Main Results:

    • A brain fraction was prepared exhibiting digitalis-like activity.
    • This fraction blocked [3H]ouabain binding to Na+,K+-ATPase.
    • The fraction inhibited 86Rb+ uptake in human erythrocytes.

    Conclusions:

    • A novel endogenous substance in the brain mimics the action of digitalis glycosides.
    • This substance has the potential to regulate ionic fluxes and concentration gradients across cell membranes.

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