Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of vitrification and the superovulated environment on placental function and fetal growth in an IVF mouse model.

Molecular human reproduction·2020
Same author

Glucocorticoid regulation of amino acid transport in primary human trophoblast cells.

Journal of molecular endocrinology·2019
Same author

Apelin is a novel regulator of human trophoblast amino acid transport.

American journal of physiology. Endocrinology and metabolism·2019
Same author

OBGYN screening for environmental exposures: A call for action.

PloS one·2018
Same author

Exposure to Phthalate, an Endocrine Disrupting Chemical, Alters the First Trimester Placental Methylome and Transcriptome in Women.

Scientific reports·2018
Same author

Pharmacogenomics of 17-alpha hydroxyprogesterone caproate for recurrent preterm birth: a case-control study.

BJOG : an international journal of obstetrics and gynaecology·2017

Related Experiment Video

Updated: May 20, 2026

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
08:21

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Published on: March 16, 2017

Gene targeting in primary human trophoblasts.

F J Rosario1, Y Sadovsky, T Jansson

  • 1Center for Pregnancy and Newborn Research, Department of Obstetrics and Gynecology, University of Texas Health Science Center San Antonio, Mail Code 7836, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA.

Placenta
|July 27, 2012
PubMed
Summary

Researchers explore gene modulation in primary human trophoblasts, essential for understanding pregnancy complications. Current methods like viral vectors and RNA interference (RNAi) show promise but require optimization to minimize risks for future gene therapies.

More Related Videos

siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts
09:57

siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

Published on: September 20, 2016

Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants
08:26

Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants

Published on: August 31, 2016

Related Experiment Videos

Last Updated: May 20, 2026

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
08:21

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Published on: March 16, 2017

siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts
09:57

siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

Published on: September 20, 2016

Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants
08:26

Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants

Published on: August 31, 2016

Area of Science:

  • Reproductive Biology
  • Genetics
  • Cell Biology

Background:

  • Primary human trophoblasts are crucial for studying placental development and pregnancy disorders.
  • Investigating trophoblast gene function necessitates reliable methods for gene expression modulation.
  • Existing techniques for gene manipulation in these cells have limitations.

Purpose of the Study:

  • To review and assess current experimental tools for modulating gene expression in primary human trophoblasts.
  • To highlight the advantages and drawbacks of lipid-based transfection, viral vectors, and RNA interference (RNAi).
  • To discuss strategies for overcoming challenges like toxicity and off-target effects.

Main Methods:

  • Evaluation of lipid-based transfection methods for nucleic acid delivery.
  • Analysis of viral vectors for efficient and stable gene expression.
  • Assessment of RNA interference (RNAi) techniques, including small interfering RNA (siRNA) and microRNA, for gene silencing.
  • Discussion of strategies to mitigate off-target effects in RNAi.

Main Results:

  • Lipid-based methods offer simplicity but may cause toxicity.
  • Viral vectors provide high efficiency, low toxicity, and stable integration.
  • RNAi is powerful for gene silencing but susceptible to off-target effects.
  • Minimization strategies for RNAi off-target effects include using multiple siRNAs and chemical modifications.

Conclusions:

  • Effective tools for gene targeting in primary human trophoblasts are emerging.
  • These methods are vital for elucidating human trophoblast gene functions.
  • Advancements in gene modulation techniques may pave the way for placental gene therapy.