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Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique

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Application of solid phase microextraction on dental composite resin analysis.

Ven-Shing Wang¹, Ta-Yuan Chang, Chien-Chen Lai

¹Department of Occupational Safety & Health, China Medical University, Taichung, Taiwan.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

|July 27, 2012

Summary

A new direct immersion solid phase microextraction (DI-SPME) method accurately measures dentin monomers like TEGDMA, UDMA, and Bis-GMA in saliva. This sensitive technique simplifies sample preparation for analyzing leached resin materials.

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Area of Science:

Analytical Chemistry
Materials Science
Biomaterials Science

Background:

Dentin monomers, including triethylene glycol dimethacrylate (TEGDMA), urethane dimethacrylate (UDMA), and 2,2-bis-[4-(2-hydroxy-3-methacryloyloxypropoxy) phenyl]-propane (Bis-GMA), are high molecular weight compounds with low vapor pressure.
These monomers are utilized in dental restorative materials and can potentially leach into saliva.

Purpose of the Study:

To develop and validate a sensitive direct immersion solid phase microextraction (DI-SPME) method for quantifying dentin monomers in saliva.
To assess the method's accuracy, precision, and detection limits for TEGDMA, UDMA, and Bis-GMA.

Main Methods:

Direct immersion solid phase microextraction (DI-SPME) utilizing a polydimethylsiloxane/divinylbenzene (PDMS/DVB) fiber.
High-performance liquid chromatography (HPLC) with an optimal detection wavelength of 215 nm.
Method validation including calibration range, accuracy, precision, and detection limit determination.

Main Results:

The DI-SPME method demonstrated excellent linearity with correlation coefficients (r) > 0.998 over a calibration range of 0.30-300 μg/mL.
High accuracy (96.1-101.2% recovery) and precision (2.30-8.15% CV) were achieved for intra- and inter-day assays.
The method achieved low detection limits: 0.03 μg/mL for TEGDMA, 0.27 μg/mL for UDMA, and 0.06 μg/mL for Bis-GMA.

Conclusions:

DI-SPME is a sensitive and efficient technique for analyzing dentin monomers in saliva.
The method requires minimal sample pretreatment, making it advantageous for leaching studies of dental resin materials.