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Phosphoinositides and PIPs

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...

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Related Experiment Video

Updated: May 20, 2026

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Published on: January 6, 2016

Lipid phosphate phosphatase (LPP3) and vascular development.

H Ren¹, M Panchatcharam, P Mueller

¹The Gill Heart Institute, Division of Cardiovascular Medicine, Lexington, KY 40536-0200, USA.

Biochimica Et Biophysica Acta

|July 28, 2012

Summary

Lipid phosphate phosphatases (LPPs) are enzymes with diverse functions. LPP3 specifically plays a critical role in vascular development and is linked to coronary artery disease risk.

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Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

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Last Updated: May 20, 2026

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

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Published on: January 6, 2016

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Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

Area of Science:

Biochemistry
Molecular Biology
Cardiovascular Research

Background:

Lipid phosphate phosphatases (LPPs) are integral membrane proteins with broad substrate specificity.
Mammalian LPP enzymes (LPP1-3) exhibit overlapping in vitro activities but possess nonredundant in vivo functions.
LPP3 has been implicated in vascular development.

Purpose of the Study:

To review the evidence linking LPP3 to vascular development and disease.
To explore potential molecular mechanisms underlying LPP3's role in vascular health.
To highlight the significance of LPP3 in cardiovascular disease.

Main Methods:

Review of studies on mice with targeted inactivation of Ppap2 genes.
Analysis of large genome-wide association studies (GWAS).
Examination of existing literature on LPP enzymes and lysophospholipid metabolism.

Main Results:

Studies in mice lacking Ppap2b reveal a specific role for LPP3 in vascular development.
A single nucleotide polymorphism in PPAP2B is a novel predictor of coronary artery disease.
Evidence suggests nonredundant functions for LPP enzymes despite overlapping in vitro activities.

Conclusions:

LPP3 is a key regulator of vascular development.
Genetic variations in PPAP2B are associated with coronary artery disease risk.
Further research into LPP3's molecular mechanisms is warranted for understanding and treating vascular diseases.