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Related Concept Videos

Amperometry: Overview01:10

Amperometry: Overview

Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...

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Related Experiment Video

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High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
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Published on: April 23, 2019

Amperometric biosensor for oxalate determination in urine using sequential injection analysis.

Jose A Rodriguez1, Prisciliano Hernandez, Veronica Salazar

  • 1Chemical Research Center, Universidad Autonoma el Estado de Hidalgo, Carr. Pachuca-Tulancingo km 4.5, 42076 Pachuca, Hidalgo, Mexico. josear@uaeh.edu.mx

Molecules (Basel, Switzerland)
|July 28, 2012
PubMed
Summary
This summary is machine-generated.

This study presents a novel amperometric flow biosensor for accurate oxalate determination in urine. The developed biosensor utilizes immobilized oxalate oxidase on magnetic nanoparticles for efficient and sensitive analysis.

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Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney
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Published on: October 12, 2015

Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Electrochemistry

Background:

  • Oxalate determination in urine is crucial for diagnosing and monitoring conditions like kidney stones.
  • Existing methods for oxalate analysis can be time-consuming or require complex sample preparation.

Purpose of the Study:

  • To develop and optimize a rapid and sensitive amperometric flow biosensor for quantifying oxalate in urine samples.
  • To utilize immobilized oxalate oxidase on magnetic nanoparticles for enhanced biosensor performance.

Main Methods:

  • Enzymatic reaction of oxalate with immobilized oxalate oxidase on modified magnetic nanoparticles.
  • Magnetic retention of the modified magnetic solid on an electrode surface within a sequential injection system.
  • Amperometric detection coupled with Taguchi parameter design for system optimization (flow rate, volumes, coil length).

Main Results:

  • Linear calibration curve for oxalate detection between 3.0-50.0 mg·L⁻¹.
  • Achieved a low limit of detection of 1.0 mg·L⁻¹.
  • Demonstrated high repeatability (0.7% for 30.0 mg·L⁻¹) and validation against spectrophotometry with no significant differences.

Conclusions:

  • The developed amperometric flow biosensor offers a sensitive, repeatable, and reliable method for urine oxalate determination.
  • The use of magnetic nanoparticles and sequential injection system simplifies the analytical procedure.
  • This biosensor shows potential for routine clinical diagnostics and research applications.