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Updated: May 20, 2026
Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
Published on: March 17, 2023
Adipose triglyceride lipase (ATGL) clone, expression pattern, and regulation by different lipid sources and lipid
Xinxia Wang1, Yizhen Wang, Yongjin Li
1Institute of Feed Science, Zhejiang University, No. 388 Yuhangtang Road, Hangzhou, People's Republic of China.
Abstract:
Adipose triglyceride lipase (ATGL) was recently identified as a triglyceride (TG)-specific lipase. In this study, we first obtained from large yellow croaker fish a 1,820-bp (GenBank ID: HQ916211) ATGL cDNA fragment with a 141-bp 5'UTR, a 1,485-bp open reading frame, and a 194-bp 3'UTR. The predicted fish ATGL had 494 amino acids (GenBank ID: ADY89608) and a calculated molecular weight of 55.1 kDa. ATGL was highly expressed in liver and, to a lesser degree, in heart, muscle, and abdominal fat. ATGL gene expression was high at 4.5 g and then decreased at 157.9 g and increased again at 474.2 g. The effects of lipid levels and lipid sources on ATGL expression in vivo were also investigated. A high-lipid diet decreased ATGL expression in fish significantly (P < 0.01). Fish in soybean oil group exhibited significantly lower ATGL expression than fish in the fish oil and beef tallow groups (P < 0.01). These data enhance our understanding of ATGL in fish lipid metabolism.
