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Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring

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Published on: December 9, 2010

Biological tissue imaging with a position and time sensitive pixelated detector.

Julia H Jungmann¹, Donald F Smith, Luke MacAleese

¹FOM Institute AMOLF, Amsterdam, The Netherlands.

Journal of the American Society for Mass Spectrometry

|July 28, 2012

Summary

This study showcases a new Timepix detector for high-resolution mass spectrometric imaging of biological tissues. It enables fast, large-area analysis of biomolecules in samples like mouse testis.

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Area of Science:

Biophysics
Analytical Chemistry
Materials Science

Background:

Mass spectrometric imaging (MSI) is crucial for analyzing biomolecular spatial distributions in tissues.
Existing MSI techniques face limitations in speed, area coverage, and spatial resolution.
Active pixel detectors offer potential for improved MSI performance.

Purpose of the Study:

To demonstrate a highly parallel, active pixel detector for large-area mass spectrometric imaging.
To evaluate the detector's capabilities for analyzing biological tissue sections with high spatial resolution.
To assess the system's suitability for biomolecular analysis in complex biological samples.

Main Methods:

Integration of a Timepix detector (512 × 512 pixels) with chevron microchannel plates on a matrix-assisted laser desorption time-of-flight mass spectrometer (MALDI TOF-MS).
Acquisition of position- and time-resolved images of multiple m/z species in each measurement frame.
Testing with a peptide-grid benchmark sample and mouse testis tissue slices for validation.

Main Results:

The Timepix detector successfully registered mass-resolved images of multiple species from benchmark and tissue samples.
High spatial resolution down to 740 × 740 nm²/pixel and a resolving power of 6 μm were achieved.
Demonstrated automated, large-area imaging capabilities with potential for fast data acquisition.

Conclusions:

The Timepix detector system is applicable to biomolecular mass spectrometry imaging of biological tissues.
The system provides mass-spectral and localization information for analytes at physiological concentrations.
The detector shows significant potential for advancing fast, large-area, high-resolution MSI applications.