Related Experiment Video
Updated: May 20, 2026

Constructing an Olfactometer for Rodent Olfactory Behavior Studies
Published on: April 11, 2025
A comparison of sniff bottle staircase and olfactometer-based threshold tests
J E Hayes1, A L Jinks, R J Stevenson
1Department of Psychology, Faculty of Human Sciences, Macquarie University, Sydney, Australia.
Abstract:
Olfactometers have been gaining popularity as research tools, but they have yet to replace established testing procedures in a variety of laboratory and clinical settings, including absolute threshold tests. In this research, we designed and operated a simple olfactometer with which to assess threshold. To do this, we used a method-of-adjustment test that was compared to the three-alternative forced choice ascending sniff bottle staircase method, which is currently a standard threshold test procedure. We found that the olfactometer threshold test correlated highly with the staircase method, and that it possessed suitable test-retest reliability. The advantages of the olfactometer threshold test include faster test time and reduced cleaning and reassembly demands. Future use of the olfactometer in olfactory identification and/or detection thresholds amongst odors is also outlined.

