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Biotransformation of 2,6-diaminopurine nucleosides by immobilized Geobacillus stearothermophilus
Eliana C De Benedetti1, Cintia W Rivero, Claudia N Britos
1Laboratorio de Investigaciones en Biotecnología Sustentable, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal B1868BXD, Argentina.
Abstract:
An efficient and green bioprocess to obtain 2,6-diaminopurine nucleosides using thermophilic bacteria is herein reported. Geobacillus stearothermophilus CECT 43 showed a conversion rate of 90 and 83% at 2 h to obtain 2,6-diaminopurine-2'-deoxyriboside and 2,6-diaminopurine riboside, respectively. The selected biocatalyst was successfully stabilized in an agarose matrix and used to produce up to 23.4 g of 2,6-diaminopurine-2'-deoxyriboside in 240 h of process. These nucleoside analogues can be used as prodrug precursors or in antisense oligonucleotide synthesis.
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