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Published on: September 20, 2016
Expression patterns of chitinase produced from Paenibacillus chitinolyticus with different two culture media
Yong-Su Song1, Dong-Jun Seo, Kil-Yong Kim
1Division of Applied Bioscience and Biotechnology, Institute of Environmently Friendly Agriculture, College of Agricultural and Life Science, Chonnam National University, Gwangju 500-757, Republic of Korea.
Abstract:
To investigate the expression patterns of chitinase isozymes on native-PAGE and SDS-PAGE gels Paenibacillus chitinolyticus MP-306 was cultured on culture media with and without chitin substrate. P. chitinolyticus MP-306 had a strong chitinolytic activity on colloidal chitin medium. Chitinase isozymes of MP-306 were expressed as six bands (CN1-CN6) on native-PAGE gels and thirteen bands (CS1-CS13) on SDS-PAGE gels after incubation in chitin medium. Three bands (CN1, CN2, and CN3) of chitinase isozymes of MP-306 on native-PAGE gels were expressed as nine bands (CS1, CS2, CS3, CS4, CS5, CS6, CS8, CS10, and CS13) of chitinase isozymes on SDS-PAGE gels. Three bands (CN4, CN5, and CN6) of chitinase isozymes of MP-306 were strongly inhibited by metal ions on native-PAGE and SDS-PAGE gels.
