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VIM-2-producing multidrug-resistant Pseudomonas aeruginosa ST175 clone, Spain

Esther Viedma1, Carlos Juan, Jennifer Villa

  • 1Hospital Universitario 12 de Octubre, Madrid, Spain.

Emerging Infectious Diseases
|July 31, 2012
PubMed

Insights

A specific multidrug-resistant Pseudomonas aeruginosa clone (ST-175) rapidly increased in prevalence at a Spanish hospital. This clone, carrying resistance genes, significantly challenges treatment options for hospital-acquired infections.

Area of Science:

  • Clinical Microbiology
  • Infectious Diseases
  • Molecular Epidemiology

Background:

  • Multidrug-resistant Pseudomonas aeruginosa (MDR-PA) poses a significant threat in healthcare settings.
  • Nosocomial infections caused by MDR-PA are associated with high morbidity and mortality.
  • Surveillance and characterization of emerging MDR-PA clones are crucial for effective infection control.

Purpose of the Study:

  • To investigate the molecular epidemiology and drug resistance patterns of MDR-PA isolates from a Spanish hospital between 2007 and 2010.
  • To identify and characterize dominant MDR-PA clones contributing to the increasing prevalence.

Main Methods:

  • Molecular epidemiologic analysis including genotyping and multilocus sequence typing (MLST).
  • Drug resistance profiling of identified isolates.
  • Polymerase chain reaction (PCR) to detect specific resistance genes (blaVIM-2, blaIMP-22).

Main Results:

  • A significant increase in MDR-PA prevalence from 2.8% to 15.3% was observed between 2007 and 2010.
  • A single major clone, identified as sequence type (ST) 175, accounted for 56.8% of isolates.
  • The ST-175 clone predominantly carried the blaVIM-2 gene and exhibited resistance to multiple antibiotics, with susceptibility only to amikacin and colistin.

Conclusions:

  • The emergence and rapid dissemination of the MDR-PA ST-175 clone represent a major public health concern.
  • This clone's resistance profile compromises therapeutic options for P. aeruginosa nosocomial infections.
  • Enhanced surveillance and infection control measures are necessary to manage the spread of such high-risk clones.

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