Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Redox Titration: Iodimetry and Iodometry01:23

Redox Titration: Iodimetry and Iodometry

Iodometry and iodimetry are analytical methods used to determine the concentration of oxidizing or reducing agents using iodine. In iodometric titrations, the oxidizing analyte solution is usually acidified and treated with an excess of iodide ions, which generates an equivalent amount of iodine in equilibrium with triiodide. The released iodine is subsequently titrated directly against a standardized reducing agent. As the dilute iodine color becomes pale yellow, a few drops of freshly...
Redox Titration: Overview01:21

Redox Titration: Overview

Redox titration is a chemical analysis technique used to determine the concentration of an unknown substance by measuring the electron transfer in a redox (reduction-oxidation) reaction. The process involves gradually adding a titrant with a known concentration of an oxidizing or reducing agent, to the analyte, the solution with an unknown concentration, until reaching the endpoint, which indicates the completion of the reaction between the two substances. Ensuring the analyte is in a single...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Redox Titration: Other Oxidizing and Reducing Agents01:26

Redox Titration: Other Oxidizing and Reducing Agents

Besides iodine, other oxidizing or reducing agents can serve as titrants in redox titrations. Common oxidizing titrants include KMnO4, cerium(IV), and K2Cr2O7. The choice of oxidizing titrants depends on factors like stability, cost, analyte strength, and reaction rate between the analyte and titrant. KMnO4 is a strong oxidizing titrant that reduces from Mn(VII) to Mn(II) in a highly acidic solution, simultaneously oxidizing the analyte to a higher oxidation state. In this case, KMnO4 acts as a...
In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chemokine CCL21 promotes the pathological progression of endometriosis by regulating inflammatory cytokine expression and activating the NF-κB signaling pathway.

International immunopharmacology·2026
Same author

Combined nocturnal sleep pattern and napping duration in relation to metabolic dysfunction-associated steatotic liver disease risk and prediction in type 2 diabetes mellitus.

Diabetology & metabolic syndrome·2026
Same author

Poriae cutis triterpenes ameliorate metabolic-associated steatotic liver disease by inhibiting PXR/NLRP3 pathways.

Chinese medical journal·2026
Same author

Comment on "BMJ Publications on Interventional Techniques Do Not Meet Appropriateness Criteria of Conducting a Rapid Review: A Comprehensive Review".

Pain physician·2026
Same author

Dopamine neurons in the ventral tegmental area regulate emotional disturbances in a mouse model of acute respiratory distress syndrome.

Science bulletin·2026
Same author

Long-Range Exciton Diffusion and Efficient Hole Transfer in Molecular Non-Fullerene Acceptors with Strong Resonance Structure.

The journal of physical chemistry letters·2026

Related Experiment Video

Updated: May 20, 2026

An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay
09:47

An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay

Published on: February 1, 2019

A simple sensitive colorimetric/fluorometric probe for iodide.

Yang Zhao1, Liang Yao, Ming Zhang

  • 1State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, PR China.

Talanta
|July 31, 2012
PubMed
Summary

A novel fluorene oligomer (OCZ) with carbazole side chains effectively detects iodide ions (I(-)) using colorimetric and fluorometric methods. This probe exhibits high sensitivity and selectivity, enabling naked-eye detection at low concentrations.

More Related Videos

Iridium(III) Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
12:52

Iridium(III) Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
08:17

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

Related Experiment Videos

Last Updated: May 20, 2026

An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay
09:47

An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay

Published on: February 1, 2019

Iridium(III) Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
12:52

Iridium(III) Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
08:17

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Organic Chemistry

Background:

  • Development of sensitive and selective chemical sensors is crucial for environmental and biological monitoring.
  • Fluorene oligomers functionalized with electron-rich groups offer potential for optical sensing applications.
  • Carbazole moieties are known for their charge-transport properties and fluorescence characteristics.

Purpose of the Study:

  • To synthesize a novel fluorene oligomer, 7,7'-bis(9,9-bis(6-(9H-carbazol-9-yl)hexyl)-9H-fluoren-2-yl)-9,9,9',9'-tetrakis(6-(9H-carbazol-9-yl)hexyl)-9H,9'H-2,2'-bifluorene (OCZ).
  • To investigate the potential of OCZ as a colorimetric and fluorometric probe for iodide (I(-)) detection.
  • To evaluate the sensitivity, selectivity, and detection limits of the OCZ probe for iodide.

Main Methods:

  • Synthesis of the fluorene oligomer OCZ featuring peripheral carbazole side chains.
  • Spectroscopic analysis (UV-Vis absorption and fluorescence spectroscopy) to study the interaction of OCZ with iodide ions.
  • Colorimetric assessment of OCZ response to varying concentrations of iodide ions.

Main Results:

  • The synthesized OCZ probe demonstrated high sensitivity and selectivity for iodide (I(-)) detection.
  • Significant fluorescence quenching was observed in the presence of iodide ions.
  • Visible color changes were observed with the naked eye at a low concentration of 0.1 mM iodide, indicating excellent performance.

Conclusions:

  • The OCZ fluorene oligomer serves as an effective colorimetric and fluorometric sensor for iodide detection.
  • The probe's superior sensitivity and selectivity are attributed to the electron-rich nature of the peripheral carbazole groups.
  • OCZ represents a promising material for developing advanced iodide sensing technologies.