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Phylogeny and molecular typing of Mycoplasma agalactiae and Mycoplasma bovis by multilocus sequencing

Lucía Manso-Silván1, Virginie Dupuy, Inna Lysnyansky

  • 1CIRAD, UMR CMAEE, F-34398 Montpellier, France. lucia.manso-silvan@cirad.fr

Veterinary Microbiology
|July 31, 2012
PubMed

Insights

A new multilocus sequence typing (MLST) scheme effectively distinguishes Mycoplasma agalactiae and Mycoplasma bovis strains. This method provides robust phylogenetic analysis and precise molecular typing for epidemiological studies.

Area of Science:

  • Veterinary Microbiology
  • Molecular Phylogenetics
  • Bacterial Pathogenesis

Background:

  • Mycoplasma agalactiae and Mycoplasma bovis are significant pathogens causing similar diseases in livestock.
  • Existing methods like 16S rDNA sequencing lack the resolution for accurate differentiation and phylogenetic analysis of these closely related species.

Purpose of the Study:

  • To develop and validate a multilocus sequence typing (MLST) scheme for Mycoplasma agalactiae and Mycoplasma bovis.
  • To enable clear phylogenetic resolution, strain characterization, and molecular typing.
  • To assess the epidemiological diversity of these pathogens in different geographical regions.

Main Methods:

  • Development of an MLST scheme using housekeeping genes (fusA, gyrB, lepA, rpoB).
  • Application of the MLST scheme to a collection of M. agalactiae and M. bovis strains.
  • Inclusion of additional variable gene targets to enhance discriminatory power.
  • Phylogenetic analysis using inferred sequence data.

Main Results:

  • The developed MLST scheme clearly resolved the phylogenetic relationships between M. agalactiae and M. bovis.
  • MLST successfully characterized strains that were previously unclassifiable by serology or PCR.
  • Typing of M. agalactiae isolates revealed significant genetic diversity, with 14-17 distinct sequence types (STs) identified.
  • Analysis of strains from Turkey and Israel highlighted regional diversity and persistence of specific types over time.

Conclusions:

  • The novel MLST scheme offers a universal and accurate tool for strain characterization and global monitoring of M. agalactiae and M. bovis.
  • Incorporating more variable genetic markers enhances the scheme's utility for detailed epidemiological investigations.
  • This method is crucial for understanding the dissemination and evolution of these important veterinary pathogens.

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