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Updated: May 20, 2026

Imaging ATG9A, a Multi-Spanning Membrane Protein
Published on: June 16, 2023
Annexin A9 is a periplakin interacting partner in membrane-targeted cytoskeletal linker protein complexes
Veronika Boczonadi1, Arto Määttä
1School of Biological and Biomedical Sciences, Durham University, Durham, United Kingdom.
Abstract:
Periplakin regulates keratin organisation and participates in the assembly of epidermal cornified envelopes. A proteomic approach identified annexin A9 as a novel interacting partner for periplakin N-terminus. The presence of annexin A9 in complexes with periplakin was confirmed by immunoblotting of proteins immunoprecipitated by anti-HA or anti-annexin A9 antibodies. Both endogenous and GFP-tagged annexin A9 co-localise with endogenous periplakin and transfected periplakin N-terminus at MCF-7 cell borders and aggregate after Okadaic acid treatment. Annexin A9 and periplakin co-localise in the epidermis and annexin A9 is up-regulated in differentiating keratinocytes, but the epidermal annexin A9 expression does not require periplakin.
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