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Validated determination of primulasaponins in primula root by a high-performance-thin-layer-chromatography
Silvia A Coran1, Stefano Mulas
1Dipartimento di Scienze Farmaceutiche, Università di Firenze, Via Ugo Schiff 6, I-50019 Sesto Fiorentino, Firenze, Italy. coran@unifi.it
Abstract:
A novel HPTLC-densitometric method was developed for separation and quantitation of primulasaponin I and II in different matrices. HPTLC silica gel 60 F254(S), 20 cm × 10 cm, plates with ethyl acetate:water:formic acid (5:1:1 v/v) as the mobile phase were used. Densitometric determinations were performed in reflectance mode at 540 nm after derivatization with vanillin reagent. The method was validated giving rise to a dependable and high throughput procedure well suited to routine applications. Primulasaponins were quantified in the range of 150-450 ng with RSD of repeatability and intermediate precision between 0.8 and 1.4% and accuracy within the acceptance limits. The method was tested on commercial herbal medicinal preparations claiming to contain primula root extract.
