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Updated: May 20, 2026

Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR
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Development of swine-specific DNA markers for biosensor-based halal authentication.

M E Ali1, U Hashim, M Kashif

  • 1Institute of Nano Electronic Engineering, Universiti Malaysia, Kangar, Perlis, Malaysia. eaqubali@gmail.com

Genetics and Molecular Research : GMR
|July 31, 2012
PubMed
Summary

Researchers developed a novel nanobioprobe for detecting pork adulteration in food. This swine-specific DNA marker can identify even 1% pork in processed meat mixtures, ensuring food authenticity.

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Area of Science:

  • Food Science
  • Biotechnology
  • Genomics

Background:

  • Short DNA markers are crucial for biosensor applications due to their resilience under harsh conditions.
  • The pig (Sus scrofa) mitochondrial genome offers a target for developing specific DNA detection methods.

Purpose of the Study:

  • To design short DNA markers from the pig mitochondrial genome for biosensor-based pork detection.
  • To develop a swine-specific nanobioprobe for identifying pork adulteration in processed food products.

Main Methods:

  • In silico digestion of the swine mitochondrial genome using the AluI restriction enzyme.
  • Selection and confirmation of five potential DNA markers via NCBI blast and ClustalW alignment.
  • Integration of a labeled DNA marker onto gold nanoparticles to create a hybrid nanobioprobe.

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Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model
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Last Updated: May 20, 2026

Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR
10:16

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Published on: February 14, 2016

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model
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Published on: September 22, 2020

Main Results:

  • Five short DNA fragments were identified as suitable markers with high interspecies polymorphism and intraspecies conservatism.
  • A novel swine-specific hybrid nanobioprobe was successfully developed.
  • The nanobioprobe detected as low as 1% pork in autoclaved pork-beef mixtures without cross-species detection.

Conclusions:

  • Short DNA markers derived from mitochondrial genomes are effective for biosensor applications.
  • The developed hybrid nanobioprobe is a sensitive and specific tool for detecting pork adulteration.
  • This technology has significant implications for ensuring the authenticity of halal and kosher foods.