Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Kinetic studies on anthralin photooxidation.

K Müller1, R C Kanner, C S Foote

  • 1Department of Chemistry and Biochemistry, University of California, Los Angeles 90024.

Photochemistry and Photobiology
|September 1, 1990
PubMed
Summary

The photooxidation of anthralin is driven by its anionic form, not the neutral drug. This research quantifies the reaction rate, crucial for understanding anthralin's stability and drug interactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial.

Accounts of chemical research·2016
Same author

Characterization of a theta plasmid replicon with homology to all four large plasmids of Bacillus megaterium QM B1551.

Plasmid·1998
Same author

Cell-mediated oxidation of LDL: comparison of different cell types of the atherosclerotic lesion.

Free radical research·1998
Same author

A possible role for soluble IL-6 receptor in the pathogenesis of systemic onset juvenile chronic arthritis.

Cytokine·1998
Same author

Ilex aquifolium: protection against enzymatic and non-enzymatic lipid peroxidation.

Planta medica·1998
Same author

Kinetics and yield of singlet oxygen photosensitized by hypericin in organic and biological media.

Photochemistry and photobiology·1998

Area of Science:

  • Photochemistry
  • Chemical kinetics
  • Pharmacology

Background:

  • Anthralin is an antipsoriatic drug.
  • Understanding drug photooxidation is vital for stability and efficacy.
  • Singlet oxygen (1O2) is a reactive oxygen species implicated in photodegradation.

Purpose of the Study:

  • To investigate the photooxidation mechanism of anthralin.
  • To determine the reactive species involved in anthralin photodegradation.
  • To quantify the rate constants for singlet oxygen deactivation by anthralin.

Main Methods:

  • Direct observation of singlet oxygen luminescence at 1.27 microns.
  • Luminescence quenching studies in deuterated buffer systems.
  • Competition experiments using tetramethylethylene in acetonitrile.

Main Results:

  • The rate of singlet oxygen deactivation increases at higher pH, indicating the trihydroxyanthracene anion is the reactive species.
  • The rate constant for singlet oxygen deactivation by the anthralin anion was determined to be 3.0 x 10^8 M^-1 s^-1.
  • The neutral anthrone form of anthralin is unreactive towards singlet oxygen, with a rate constant of 2.8 x 10^4 M^-1 s^-1 in benzene-d6.

Conclusions:

  • Anthralin photooxidation is primarily mediated by its anionic form.
  • The kinetic data provide a quantitative basis for understanding anthralin's photosensitivity.
  • This study clarifies the role of singlet oxygen in anthralin degradation.

Related Experiment Videos