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Related Experiment Video

Updated: May 20, 2026

A Model of Chronic Nutrient Infusion in the Rat

A Model of Chronic Nutrient Infusion in the Rat

Published on: August 14, 2013

Impaired autophagic function in rat islets with aging.

Yu Liu¹, Suozhu Shi, Zhaoyan Gu

¹Department of Geriatric Endocrinology, Chinese PLA General Hospital, 28 Fuxing Road, Beijing, 100853, China.

Age (Dordrecht, Netherlands)

|July 31, 2012

Summary

Aging impairs pancreatic beta-cell function and autophagy, crucial for degrading cellular waste. This study in Wistar rats links declining autophagy to age-related beta-cell dysfunction and potential type 2 diabetes development.

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Last Updated: May 20, 2026

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Published on: August 14, 2013

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Development and Validation of a Methodology for Establishing Obese Rat Models with Typical Fatty Pancreas

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Published on: November 11, 2025

Area of Science:

Cellular biology
Endocrinology
Gerontology

Background:

Type 2 diabetes is linked to decreased pancreatic beta-cell function and mass, with incidence rising with age.
Autophagy, a cellular degradation process, is vital for maintaining cell health and is implicated in aging and age-related diseases.
Autophagy may be essential for preserving the structure, mass, and function of pancreatic beta-cells.

Purpose of the Study:

To investigate the impact of aging on pancreatic beta-cell function and autophagy in Wistar rats.
To examine age-related changes in autophagy markers and indicators of cellular damage in pancreatic islets.

Main Methods:

Comparison of pancreatic islets from young (4-month), adult (14-month), and old (24-month) male Wistar rats.
Analysis of protein expression for autophagy markers (LC3/Atg8, Atg7, Lamp-2, beclin-1/Atg6) and aggregate markers (p62/SQSTM1).
Assessment of oxidative DNA damage (8-hydroxydeoxyguanosine) and mitochondrial integrity via electron microscopy.

Main Results:

Beta-cell function progressively declined with age in Wistar rats.
Autophagy markers (LC3/Atg8, Atg7, Lamp-2) decreased with age, indicating reduced autophagic degradation.
Increased p62/SQSTM1, polyubiquitin aggregates, oxidative DNA damage, and mitochondrial abnormalities were observed in aged islets.

Conclusions:

Pancreatic beta-cell and autophagic function decline concurrently with aging in Wistar rats.
Impaired autophagy in aged islets may lead to protein aggregate accumulation and compromised mitochondria, reducing beta-cell function.
Dysfunctional islet autophagy during aging is a potential mechanism contributing to type 2 diabetes development.