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Updated: May 20, 2026
Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
Published on: July 19, 2019
Establishment and characterization of a new human gallbladder carcinoma cell line
Shinichi Sekine1, Yutaka Shimada, Takuya Nagata
1Department of Surgery and Science, University of Toyama, 2630 Sugitani, Toyama-city, Toyama, 930-0194, Japan. sekky@med.u-toyama.ac.jp
Background:
Prognosis for patients with gallbladder carcinoma (GBC) is poor and the standard treatment for GBC has not yet been established.
Materials And Methods:
We established the human GBC cell line TYGBK-1, from a patient with papillary, tubular adenocarcinoma.
Results:
The doubling time was 48 hours. This cell line has a missense mutation of p53 and no mutation of the K-RAS gene. This cell line was transplantable to nude mice. We characterized the sensitivity of TYGBK-1 to gemcitabine. We also examined the association of two gemcitabine-related genes (deoxycytidine kinase, dCK, and Hu antigen R, HuR). Among four GBC cell lines (TYGBK-1, NOZ, G-415, TGBC2TKB), TYGBK-1 and NOZ exhibited sensitivity to gemcitabine. Furthermore, these cells expressed both dCK and HuR mRNA, rather than gemcitabine-resistant cells.
Conclusion:
The newly established GBC cell line TYGBK-1, may represent an effective tool for development of chemotherapeutic treatment for GBC.
