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Updated: May 20, 2026
Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes
Published on: September 18, 2017
Effects of Salusin-β on action potential and ionic currents in ventricular myocytes of rats
An-Jing Ren1, Qing Liang, Rong Wang
1Department of Pathophysiology, Second Military Medical University, Shanghai, China. renanjing@gmail.com
Aim:
Salusin-β is a regulatory peptide that exerts negative inotropic effect on ventricular muscle, but its electrophysiological effects on ventricular myocytes are still unknown.
Methods:
Action potential and channel currents such as sodium current (I(N) (a) ), transient outward potassium current (I(to) ), steady-state potassium current (I(sus) ), sodium-calcium exchange current (I(N) (aCa) ) and inward rectifier potassium current (I(K) (1) ) were measured in ventricular myocytes isolated from 12 to 16 weeks rats by whole-cell voltage-clamp techniques.
Results:
Salusin-β dose-dependently shortened the duration of action potential in rat ventricular myocytes. Furthermore, salusin-β significantly inhibited I(N) (aCa) and increased I(to) , but did not affect I(N) (a) , I(sus) and I(K) (1) .
Conclusion:
These results suggest that the effect of salusin-β on action potential may be partly attributed to a decrease in inward currents and an increase in outward currents.
