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Related Experiment Video

Updated: May 20, 2026

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

Published on: April 18, 2013

LSI-based amperometric sensor for bio-imaging and multi-point biosensing.

Kumi Y Inoue¹, Masahki Matsudaira, Reyushi Kubo

¹Micro System Integration Center, Tohoku University, 519-1176 Aramaki-Aza-Aoba, Aoba-ku, Sendai, 980-0845, Japan. inoue@bioinfo.che.tohoku.ac.jp

|August 1, 2012

Summary

This summary is machine-generated.

We developed Bio-LSI, a novel electrochemical biosensor with 400 measurement points for high-resolution bio-imaging. This platform offers a wide dynamic range and real-time monitoring of biological molecules, advancing diagnostics and biochemical analysis.

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Area of Science:

Electrochemistry
Biosensor Technology
Bio-imaging

Background:

Existing biosensing platforms often lack multi-point capabilities and high spatial resolution.
There is a need for integrated systems capable of real-time electrochemical bio-imaging.

Purpose of the Study:

To develop and characterize Bio-LSI, a novel large-scale integration (LSI)-based amperometric sensor.
To demonstrate its utility for multi-point biosensing and electrochemical bio-imaging.

Main Methods:

Designed a CMOS sensor chip with 400 unit cells (20x20 array) featuring in-pixel operational amplifiers and switched-capacitor converters.
Implemented a system with external circuitry, data acquisition control, and a DC power supply.
Utilized Osmium-Horseradish Peroxidase (Os-HRP) film for sensing and demonstrated imaging of glucose oxidase (GOx)-catalyzed glucose oxidation.

Main Results:

Achieved a wide dynamic current range from ±1 pA to ±100 nA.
Demonstrated high spatial resolution (250 μm) and adaptable temporal resolution (18-125 ms/400 points).
Showcased real-time imaging of hydrogen peroxide concentration changes and glucose oxidation via GOx.

Conclusions:

Bio-LSI offers a promising platform for electrochemical bio-imaging and multi-point biosensing.
Its design enables lower detection limits and a broad detection range, suitable for various analytical applications.
The system is applicable to diagnostics, environmental monitoring, and fundamental biochemistry research.