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Related Concept Videos

Inflammatory Response I: Vascular and Cellular01:30

Inflammatory Response I: Vascular and Cellular

The inflammatory response is the body's defense against infection, injury, or irritation from bacteria, trauma, toxins, or heat. Inflammation helps locate and destroy pathogens and remove damaged tissue elements to heal the body. During this initial phase, fluid, blood products, and nutrients migrate to the injured area, resulting in redness, heat, swelling, ache, and loss of function. Moreover, signs of systemic inflammation include fever, increased WBC count, malaise, anorexia, nausea,...

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Related Experiment Video

Updated: May 20, 2026

On-Chip Endothelial Inflammatory Phenotyping
12:43

On-Chip Endothelial Inflammatory Phenotyping

Published on: July 21, 2012

On-chip endothelial inflammatory phenotyping.

J Sherrod DeVerse1, Keith A Bailey, Greg A Foster

  • 1Department of Biomedical Engineering, University of California, Davis, USA.

Journal of Visualized Experiments : Jove
|August 1, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces a novel lab-on-a-chip device to precisely measure inflammation in human endothelial cells and monocytes. This technology aids in understanding atherosclerosis development and guiding risk assessment for metabolic abnormalities.

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Related Experiment Videos

Last Updated: May 20, 2026

On-Chip Endothelial Inflammatory Phenotyping
12:43

On-Chip Endothelial Inflammatory Phenotyping

Published on: July 21, 2012

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
12:50

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Published on: September 15, 2017

Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality
10:56

Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality

Published on: May 5, 2022

Area of Science:

  • Biomedical Engineering
  • Cardiovascular Research
  • Cellular Biology

Background:

  • Metabolic abnormalities drive systemic inflammation, leading to endothelial dysfunction and potentiating atherosclerosis.
  • Current clinical methods lack direct assessment of early endothelial functional changes for risk stratification.
  • Understanding the interplay between local hemodynamics and inflammation is crucial for elucidating atherosclerosis's spatial distribution.

Purpose of the Study:

  • To develop and validate a microfluidic platform for quantitatively assessing metabolic perturbations in endothelial cell and monocyte inflammatory responses.
  • To investigate the mechanisms of inflammation under controlled shear stress conditions relevant to atherogenesis.
  • To provide a tool for early detection of endothelial dysfunction and personalized therapeutic strategies.

Main Methods:

  • Microfabrication of vascular mimetic microfluidic chambers (VMMC) using soft lithography.
  • Culturing human aortic endothelial cells (HAEC) within VMMC for direct imaging of inflammatory events.
  • Assaying monocytic cell (THP-1) rolling and arrest on HAEC under varying shear conditions and inflammatory cytokine (TNF-α) activation.

Main Results:

  • The VMMC platform successfully quantified cytokine-, lipid-, and RAGE-induced inflammation in HAEC.
  • Demonstrated the ability to study shear-dependent monocytic cell adhesion to activated endothelial monolayers.
  • Provided a quantitative method to assess endothelial cell inflammatory responses under controlled hemodynamic conditions.

Conclusions:

  • The lab-on-a-chip VMMC approach offers a powerful tool for mechanistic studies of endothelial inflammation.
  • This technology can provide insights into atherosusceptibility linked to metabolic risk factors.
  • Enables direct assessment of endothelial function to guide clinical risk stratification and therapeutic interventions.