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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
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Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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Related Experiment Video

Updated: May 20, 2026

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes
05:07

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Published on: November 7, 2025

easyRNASeq: a bioconductor package for processing RNA-Seq data.

Nicolas Delhomme1, Ismaël Padioleau, Eileen E Furlong

  • 1Genome Biology Computational Support, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany. delhomme@embl.de

Bioinformatics (Oxford, England)
|August 1, 2012
PubMed
Summary
This summary is machine-generated.

easyRNASeq simplifies RNA sequencing data processing in R. This package streamlines complex analyses by integrating multiple Bioconductor tools, making RNA-Seq analysis more accessible.

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AQRNA-seq for Quantifying Small RNAs
05:12

AQRNA-seq for Quantifying Small RNAs

Published on: February 2, 2024

Related Experiment Videos

Last Updated: May 20, 2026

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes
05:07

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Published on: November 7, 2025

AQRNA-seq for Quantifying Small RNAs
05:12

AQRNA-seq for Quantifying Small RNAs

Published on: February 2, 2024

Area of Science:

  • Bioinformatics
  • Computational Biology
  • Genomics

Background:

  • RNA sequencing (RNA-Seq) is a standard for gene expression profiling.
  • Numerous R packages exist for RNA-Seq data loading and differential expression analysis.
  • Complex processing steps between data loading and analysis require intricate package interplay.

Purpose of the Study:

  • To develop an R package simplifying RNA sequencing data processing.
  • To provide a user-friendly interface for complex RNA-Seq analysis workflows.

Main Methods:

  • Development of the 'easyRNASeq' R package.
  • Integration of multiple Bioconductor packages into a single functionality.
  • Implementation in R (version 2.15) and availability via Bioconductor (version 2.10).

Main Results:

  • The 'easyRNASeq' package simplifies the processing of RNA sequencing data.
  • It hides the complex interplay of underlying packages behind a single function.
  • Facilitates more accessible and efficient RNA-Seq data analysis.

Conclusions:

  • 'easyRNASeq' enhances the usability of RNA sequencing analysis in R.
  • The package reduces the technical barrier for researchers analyzing gene expression data.
  • Promotes wider adoption and application of RNA-Seq in biological research.