Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long-term remission and monocyclic course in Still's disease patients starting canakinumab early: data from the international AIDA network registry.

Seminars in arthritis and rheumatism·2026
Same author

AIRRWAS integrates TCR-microbiome data to reveal how the gut microbiome shapes the circulating and mucosal T cell repertoire.

Cell reports·2026
Same author

The critical role of accurate neoplastic cell percentage (NCP) assessment: investigating targeted training strategies for pulmonary biopsy and cytology specimens.

Virchows Archiv : an international journal of pathology·2026
Same author

Increasing Transfection of Human Monocyte-Derived Dendritic Cells by Optimizing Lipid Nanoparticle Ionizable Lipid and mRNA Uridine Modification.

Pharmaceutics·2026
Same author

Application of machine learning techniques to explore the occurrence of macrophage activation syndrome in Still's disease: results from the GIRRCS AOSD Study Group and the AIDA Network Still's Disease Registry.

Frontiers in immunology·2026
Same author

HER2 expression and <i>HER2</i> gene amplification in thymic epithelial tumors: a narrative review.

Mediastinum (Hong Kong, China)·2026

Related Experiment Video

Updated: May 20, 2026

Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples
12:34

Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples

Published on: July 6, 2014

Sampling site matters when counting lymphocyte subpopulations.

Benson Ogunjimi1, Dieter Peeters, Niel Hens

  • 1Centre for Health Economics Research and Modeling Infectious Diseases, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium. benson.ogunjimi@ua.ac.be

Plos One
|August 1, 2012
PubMed
Summary
This summary is machine-generated.

Blood sampling site impacts immune cell counts. Distal sites (radial artery, dorsal hand veins) show higher naive T cells than proximal antecubital veins, while effector memory T cells are reversed. This affects immunological research and clinical practice.

More Related Videos

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
08:44

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Published on: September 7, 2022

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
14:45

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Published on: September 16, 2012

Related Experiment Videos

Last Updated: May 20, 2026

Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples
12:34

Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples

Published on: July 6, 2014

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
08:44

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Published on: September 7, 2022

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
14:45

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Published on: September 16, 2012

Area of Science:

  • Immunology
  • Hematology
  • Clinical Research

Background:

  • Blood analyses commonly use antecubital venipuncture.
  • The validity of antecubital veins as a proxy for other sites is routinely assumed.

Purpose of the Study:

  • To test if antecubital veins are a reliable proxy for blood cell counts from other sites.
  • To investigate differences in cell counts between arterial, dorsal hand venous, and antecubital venous blood.

Main Methods:

  • Analyzed blood cell counts from radial artery, dorsal hand veins, and antecubital veins.
  • Utilized hematocytometry and flow cytometry.
  • Included 18 healthy volunteers in the study.

Main Results:

  • Greatest cell count differences were between distal (radial artery, dorsal hand veins) and proximal (antecubital veins) sites, not arterial vs. venous.
  • Naïve T cell counts were higher in distal samples; effector memory T cell counts were higher in proximal samples.
  • High correlations were observed between different sampling sites despite observed differences.

Conclusions:

  • Antebcubital veins are not a perfect proxy for all blood sampling sites, with significant differences in T cell subsets.
  • Findings are critical for interpreting immunological research and informing clinical practice.
  • Results suggest a potential role for interval lymph nodes in lymphocyte trafficking.