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Mucosal dysfunction and damage induced by platelet activating factor (PAF)
1Department of Otorhinolaryngology, Yamanashi College of Medicine, Japan.
Acta Oto-Laryngologica
|November 1, 1990
Summary
Platelet-activating factor (PAF) was found to inhibit human nasal mucosa ciliary activity in a dose-dependent manner. This study indicates PAF has a cytotoxic effect on paranasal sinus mucosa.
Area of Science:
- Immunology
- Cell Biology
- Otolaryngology
Background:
- Platelet-activating factor (PAF) is a potent inflammatory mediator.
- The role of PAF in nasal mucosa function requires further elucidation.
Purpose of the Study:
- To investigate the in vitro effects of PAF on human nasal mucosa.
- To assess the impact of PAF on ciliary activity and morphology.
Main Methods:
- Human ethmoid sinus mucosa was cultured in vitro.
- Ciliary activity was measured photoelectrically and observed via microscopy.
- Morphological changes were examined using light and electron microscopy.
Main Results:
- PAF inhibited human nasal mucosa ciliary activity in a time- and dose-dependent manner (10^-6M to 10^-10M).
- Edema, cell exfoliation, and desquamation of the epithelial layer increased with time.
- Ultrastructural alterations preceded the inhibition of ciliary activity.
Conclusions:
- PAF exhibits a cytotoxic effect on human paranasal sinus mucosa.
- These findings suggest PAF plays a role in sinonasal inflammatory conditions.