[Construction and application of atropine flow-through sensor in flow injection analysis]

Area of Science:

• Analytical Chemistry
• Electrochemistry
• Sensor Technology

Background:

• Atropine determination is crucial for pharmaceutical analysis.
• Existing methods for atropine quantification can be time-consuming or lack sensitivity.
• Development of rapid and selective sensors is needed for efficient analysis.

Purpose of the Study:

• To develop and characterize a new flow-through sensor for atropine.
• To evaluate the sensor's performance in terms of Nernstian response, selectivity, and accuracy.
• To apply the sensor in flow injection analysis for real-world sample matrices.

Main Methods:

• Fabrication and testing of a novel flow-through potentiometric sensor.
• Determination of Nernstian slope and response range for atropine.
• Assessment of interference from common chemical compounds.
• Validation using direct potentiometry and flow injection analysis (FIA).

Main Results:

• The sensor exhibited a Nernstian response for atropine (54 ± 1 mV/decade) from 0.02-200 mmol/L at pH 5-8.
• Minimal interference was observed, with only four compounds showing significant cross-reactivity.
• Direct potentiometry yielded 99.2% average recovery and 1.3% relative standard deviation.
• FIA enabled rapid analysis of atropine, anisodamine, scopolamine, and belladonna preparations at 60-100 samples/h.

Conclusions:

• The developed flow-through sensor offers a sensitive and selective method for atropine determination.
• The sensor is suitable for rapid analysis in pharmaceutical and botanical samples using FIA.
• This technology provides a valuable tool for efficient quality control and research.