Related Experiment Video
Updated: May 20, 2026

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging
Published on: July 16, 2021
Small molecules that regulate zymosan phagocytosis of macrophage through deactivation of Rho GTPases
Joon Seok Bang1, Young Jin Kim, Jiho Song
1Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea.
Abstract:
Phagocytosis and subsequent degradation of pathogens by macrophages play a pivotal role in host innate immune response to microbial infections. To find small molecule regulators for the investigation of complicated phagocytic process, we screened our in-house chemical library and found chemicals which inhibit phagocytosis of zymosan by macrophages. A representative compound 5a reduced phagocytosis of zymosan in both peritoneal macrophages and RAW264.7 cells in a dose-dependent manner. Treatment of 5a led to downregulate the key regulators of phagocytosis, Rac1, Rac2 and Cdc42, and slightly reduced phosphorylation of Akt by zymosan.
More Related Videos
09:22High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization
Published on: November 27, 2014
11:48Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
Published on: May 31, 2018
Related Concept Videos
Small GTPases - Ras and Rho
Three regulatory proteins control their activity:
Phagocytosis of Apoptotic Cells
Normal cells contain receptors that prevent them from being recognized by phagocytes.
Amplifying Signals via Enzymatic Cascade
Cell Polarization by Rho Proteins
Gene Regulation in Microbial Communities: Quorum Sensing
Role of Matrix Metalloproteases in Degradation of ECM
A...