Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Southern Blot

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

In-situ Hybridization

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Innovations in Worksite Diagnosis of Urinary Tract Infections and the Occupational Health Nurse.

Workplace health & safety·2019

Same author

Label-free detection of DNA hybridization with a compact LSPR-based fiber-optic sensor.

The Analyst·2017

Same author

Novel stem-loop probe DNA arrays: detection of specific acetotrophic 16S ribosomal RNA signatures.

Analytical biochemistry·2012

Same author

Chaotic mixer improves microarray hybridization.

Analytical biochemistry·2004

Same author

Effects of relative humidity and buffer additives on the contact printing of microarrays by quill pins.

Analytical biochemistry·2003

Same author

FTIR/ATR study of protein adsorption and brushite transformation to hydroxyapatite.

Biomaterials·2002

Search research articles

Related Experiment Video

Updated: May 20, 2026

Studying DNA Looping by Single-Molecule FRET

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

Dendron-modified surfaces provide an ideal environment for stem-loop DNA probes.

Jonas Boateng¹, Joel Peek, Robert Zahorchak

¹Department of Chemical Engineering, University of Alabama in Huntsville, Huntsville, AL 35899, USA.

Analytical Biochemistry

|August 2, 2012

Summary

Stem-loop DNA probes (SLPs) immobilized on dendron-modified surfaces improve nucleic acid detection. This novel approach enhances specificity and sensitivity, enabling detection of targets at 1pM concentrations.

More Related Videos

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells

Published on: March 20, 2021

Related Experiment Videos

Last Updated: May 20, 2026

Studying DNA Looping by Single-Molecule FRET

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells

Published on: March 20, 2021

Area of Science:

Biotechnology
Molecular Biology
Surface Chemistry

Background:

DNA microarrays are crucial for genetic analysis, but their specificity and sensitivity are limited by probe design and surface interactions.
Linear DNA probes can be less specific than stem-loop DNA probes (SLPs).
Immobilizing SLPs on surfaces can disrupt their native structure, hindering performance.

Purpose of the Study:

To develop an improved method for immobilizing SLPs on surfaces for enhanced nucleic acid detection.
To investigate the use of dendron-modified surfaces for SLP immobilization.
To assess the sensitivity and specificity of SLPs on these novel surfaces.

Main Methods:

Dendron-modified surfaces with uniform spacing of aldehyde functionalities were prepared.
Stem-loop DNA probes (SLPs) were immobilized onto these functionalized surfaces.
The ability of immobilized SLPs to detect target nucleic acids was evaluated using varying concentrations.

Main Results:

Dendron-modified surfaces provided a suitable substrate for SLP immobilization, preserving their structure.
The uniform spacing created a solution-like environment, facilitating probe-target interactions.
The system achieved sensitive detection of nucleic acid targets down to 1 picomolar (pM) concentrations.

Conclusions:

Dendron-modified surfaces offer an effective platform for immobilizing SLPs, enhancing their performance in nucleic acid detection.
This approach significantly improves the sensitivity and specificity of DNA microarrays.
The developed method holds promise for advanced molecular diagnostics and research applications.