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Related Experiment Video

Updated: May 19, 2026

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia
09:57

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Published on: March 5, 2018

Risk-based classification of leukemia by cytogenetic and multiplex molecular methods: results from a multicenter

C D Gocke, J Mason, L Brusca

    Blood Cancer Journal
    |August 2, 2012
    PubMed
    Summary

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    EClinicalMedicine·2022

    This study validates a new multiplex molecular assay for detecting leukemia fusion transcripts, showing high accuracy and sensitivity. This method aids in leukemia diagnosis, prognosis, and monitoring, complementing traditional cytogenetics.

    Area of Science:

    • Hematology
    • Molecular Diagnostics
    • Oncology

    Background:

    • Accurate diagnosis and prognosis of leukemia rely on identifying cytogenetic abnormalities.
    • Current methods may not capture all relevant molecular markers for comprehensive patient management.

    Purpose of the Study:

    • To perform the first multicenter validation of a multiplex molecular assay for 12 key leukemia fusion transcripts.
    • To compare the performance of this assay against standard cytogenetic methods and reference molecular tests.

    Main Methods:

    • Multiplex reverse transcription-polymerase chain reaction (RT-PCR) followed by liquid bead array detection.
    • Evaluation on 280 adult and pediatric acute or chronic leukemia samples with diverse clinical presentations.
    • Comparison with cytogenetics and independent reference molecular tests.
    Keywords:
    RT-PCRdiagnosisleukemiamolecular classificationmultiplexprognosis

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    Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation
    12:05

    Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation

    Published on: November 3, 2018

    Related Experiment Videos

    Last Updated: May 19, 2026

    Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia
    09:57

    Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

    Published on: March 5, 2018

    Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation
    12:05

    Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation

    Published on: November 3, 2018

    Main Results:

    • High overall agreement (>98.5%) across four clinical sites.
    • Excellent concordance with cytogenetic methods and high qualitative agreement (98.7%) with reference molecular tests.
    • Demonstrated method sensitivity of 1% for detecting low blast count or relapsed cases.

    Conclusions:

    • The multiplex RT-PCR assay is a rapid, flexible, and accurate method for leukemia molecular profiling.
    • This assay complements cytogenetic methods, providing valuable data for diagnosis, prognosis, and molecular monitoring.
    • Potential for enhanced clinical sensitivity by incorporating rare variants and additional transcripts.