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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Method to quantify cytokines and chemokines in mouse brain tissue using Bio-Plex multiplex immunoassays.

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Bio-Rad's Bio-Plex® suspension array system, xMAP technology overview.

Brett Houser1

  • 1Bio-Rad Laboratories, Inc., Hercules, California 94547, USA. Brett_houser@Bio-Rad.com

Archives of Physiology and Biochemistry
|August 3, 2012
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Summary
This summary is machine-generated.

The Bio-Plex system uses xMAP technology for multiplex analysis, allowing simultaneous detection of up to 100 analytes. This method provides more data from less sample, faster than traditional assays.

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Area of Science:

  • Biotechnology
  • Immunology
  • Analytical Chemistry

Background:

  • Traditional immunoassays like ELISA are time-consuming and require larger sample volumes.
  • Multiplex analysis offers a more efficient approach to detecting multiple analytes simultaneously.
  • The xMAP technology provides a platform for high-throughput, bead-based multiplex assays.

Purpose of the Study:

  • To describe the Bio-Plex system and its underlying xMAP technology.
  • To highlight the advantages of multiplex analysis over traditional immunoassay methods.
  • To explain the mechanism of analyte capture and detection using bead sets.

Main Methods:

  • Utilizes xMAP technology for multiplexing up to 100 analytes.
  • Employs antibody-sandwich assays for analyte detection.
  • Uses differentially detectable bead sets as capture substrates in solution.
  • Employs fluorescent methods for signal detection and quantification.

Main Results:

  • Enables simultaneous identification and quantification of multiple analytes in a single sample.
  • Provides more information from smaller sample volumes compared to traditional methods.
  • Reduces assay time and resource requirements.

Conclusions:

  • The Bio-Plex system with xMAP technology offers a powerful tool for efficient multiplex analysis.
  • This technology enhances research capabilities by enabling comprehensive analyte profiling.
  • It represents a significant advancement over conventional immunoassay techniques.