Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Magainin analogs effective against pathogenic protozoa.

C M Huang1, H C Chen, C H Zierdt

  • 1Department of Pathology, Thomas Jefferson University Hospital, Philadelphia, Pennsylvania 19107.

Antimicrobial Agents and Chemotherapy
|September 1, 1990
PubMed
Summary

Magainin B and G analogs show potent antiprotozoan activity against Blastocystis hominis, Entamoeba histolytica, and Trypanosoma cruzi. Their effectiveness correlates with alpha-helix content, highlighting potential therapeutic applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Clinical practice, quality control, and training in robot-assisted gastric cancer surgery].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2026
Same author

[Long-term oncological safety of robotic total gastrectomy for locally advanced proximal gastric cancer: a 5-year noninferiority comparison based on the FUGES-014 study].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2025
Same author

[Prognostic significance of textbook outcome in advanced gastric patients who underwent neoadjuvant chemotherapy followed by surgical resection].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2024
Same author

[Quality control of gastric resection range in laparoscopic locally advanced gastric cancer].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2024
Same author

[Establishment of a nomogram prediction model using common preoperative indicators for early weight loss after laparoscopic sleeve gastrectomy].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2023
Same author

[Treatment and outcome of a young female patient with severe aortic stenosis and complex coronary disease associated with familial hypercholesterolemia: a case report].

Zhonghua xin xue guan bing za zhi·2023

Area of Science:

  • Antimicrobial Peptides
  • Parasitology
  • Molecular Biology

Background:

  • Protozoan infections caused by Blastocystis hominis, Entamoeba histolytica, and Trypanosoma cruzi pose significant global health challenges.
  • Development of novel therapeutic agents against these protozoa is crucial, especially with increasing drug resistance.
  • Magainins, a class of antimicrobial peptides, have demonstrated broad-spectrum activity, but their efficacy against specific protozoa requires detailed investigation.

Purpose of the Study:

  • To evaluate the in vitro antiprotozoan activity of various magainin analogs.
  • To determine the correlation between the alpha-helix content of magainin analogs and their protozoan-killing efficacy.
  • To identify specific magainin analogs effective against Blastocystis hominis, Entamoeba histolytica, and Trypanosoma cruzi.

Related Experiment Videos

Main Methods:

  • In vitro assessment of magainin analogs' activity using protozoan morphological integrity and motility assays.
  • Comparative analysis of antiprotozoan efficacy across different magainin analogs (B, G, H).
  • Quantification of alpha-helix content in the tested magainin analogs.

Main Results:

  • Antiprotozoan activity varied among magainin analogs, with the order of efficacy being magainin B > G > H.
  • This activity order directly correlated with the measured alpha-helix content of the respective analogs.
  • Magainin B and magainin G demonstrated significant effectiveness against Blastocystis hominis, Trypanosoma cruzi, and Entamoeba histolytica.

Conclusions:

  • Magainin B and G are potent antiprotozoan agents effective against Blastocystis hominis, Entamoeba histolytica, and Trypanosoma cruzi in vitro.
  • The alpha-helical structure of magainin analogs is a key determinant of their antiprotozoan activity.
  • These findings suggest that magainin analogs, particularly B and G, hold promise as lead compounds for developing new anti-parasitic therapies.