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Updated: May 19, 2026

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
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Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

Trapping DNA near a solid-state nanopore.

Dimitar M Vlassarev1, Jene A Golovchenko

  • 1Department of Physics, Harvard University, Cambridge, Massachusetts, USA.

Biophysical Journal
|August 3, 2012
PubMed
Summary
This summary is machine-generated.

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Voltage-biased solid-state nanopores can trap double-stranded DNA (dsDNA) molecules. This trapping event causes a decrease in ionic current, differentiating it from translocation events which increase current.

Area of Science:

  • Nanotechnology
  • Biophysics
  • Molecular Biology

Background:

  • Solid-state nanopores are crucial for single-molecule analysis.
  • Controlling molecule behavior within nanopores is essential for advanced applications.
  • Understanding DNA-nanopore interactions is key to developing novel biosensing technologies.

Purpose of the Study:

  • To demonstrate transient localization of double-stranded DNA (dsDNA) within voltage-biased solid-state nanopores.
  • To differentiate the ionic current signature of DNA localization from translocation.
  • To validate experimental observations with computational modeling.

Main Methods:

  • Utilizing voltage-biased solid-state nanopores in an electrolyte solution.
  • Applying electric fields to interact with dsDNA molecules.

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Published on: January 15, 2013

Related Experiment Videos

Last Updated: May 19, 2026

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

Monitoring Protein Adsorption with Solid-state Nanopores
08:51

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

  • Monitoring ionic current changes through the nanopore.
  • Performing finite-element modeling to simulate dsDNA behavior.

    • Main Results:

    • Transient localization of dsDNA was achieved by trapping a non-end segment across the nanopore orifice.
    • dsDNA trapping resulted in a measurable decrease in ionic current.
    • Translocating dsDNA molecules under similar conditions caused an ionic current increase.
    • Finite-element modeling results corroborated the experimental observations.

    Conclusions:

    • Voltage-biased nanopores offer a method for transiently localizing dsDNA.
    • Distinct ionic current changes can differentiate DNA localization from translocation.
    • The study provides a foundation for manipulating DNA at the nanoscale using nanopore technology.