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Related Experiment Video

Updated: May 19, 2026

In Vitro Analysis of E3 Ubiquitin Ligase Function

In Vitro Analysis of E3 Ubiquitin Ligase Function

Published on: May 14, 2021

Ubiquitin E3 ligase A20 contributes to maintaining epithelial barrier function.

Peixin Huang¹, Xiao-Rui Geng, Gui Yang

¹Department of Gastroenterology, the Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology

|August 3, 2012

Summary

Ubiquitin E3 ligase A20 (A20) is crucial for epithelial barrier function. A20 facilitates allergen degradation, preventing immune responses and maintaining gut homeostasis.

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Last Updated: May 19, 2026

In Vitro Analysis of E3 Ubiquitin Ligase Function

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Published on: May 14, 2021

Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor

Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor

Published on: February 10, 2014

Area of Science:

Immunology
Cell Biology
Gastroenterology

Background:

Epithelial barrier dysfunction is implicated in allergic diseases.
The precise mechanisms underlying this dysfunction require further elucidation.
Ubiquitin E3 ligase A20 (A20) is known to maintain bodily homeostasis.

Purpose of the Study:

To investigate the role of A20 in maintaining epithelial barrier integrity.
To understand how A20 influences the handling of exogenous allergens by epithelial cells.

Main Methods:

Caco-2 cell monolayers were used to study the endocytosis and degradation of ovalbumin (OVA).
A20-sufficient and A20-deficient Caco-2 cells were employed to assess A20's role in endosome-lysosome fusion.
Immunocytochemistry was utilized for visualization.

Main Results:

Caco-2 cells demonstrated endocytosis of exogenous allergens (OVA).
A20-sufficient cells degraded OVA via endosome-lysosome fusion.
A20-deficient cells allowed OVA to translocate to the basal compartment, retaining antigenicity and inducing T cell proliferation.

Conclusions:

A20 is essential for the proper fusion of endosomes and lysosomes in epithelial cells.
A20 plays a critical role in maintaining epithelial barrier function by facilitating allergen degradation.