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Published on: October 6, 2022
Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy
Shizuka Nakayama1, Yiling Luo, Jie Zhou
1Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
Chemical Communications (Cambridge, England)
|August 3, 2012
Abstract:
C-di-GMP regulates important processes involved in biofilm formation and virulence factors production in several bacteria. Herein we report a simple fluorescent strategy that allows for the detection of c-di-GMP (as low as 320 nM) using a Vc2 class I riboswitch domain as the sensing region and spinach as the fluorescent reporting module.
