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Related Experiment Video

Updated: May 19, 2026

Isolation of Human Neutrophils from Whole Blood and Buffy Coats

Isolation of Human Neutrophils from Whole Blood and Buffy Coats

Published on: September 17, 2021

Isolation of human eosinophils.

Praveen Akuthota¹, Revital Shamri¹, Peter F Weller¹

¹Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts.

Current Protocols in Immunology

|August 3, 2012

Summary

This summary is machine-generated.

This study presents a fast and reproducible method for isolating highly purified eosinophils from peripheral blood using antibody-based magnetic negative selection. The protocol ensures high purity and allows for differentiation from other leukocytes.

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Last Updated: May 19, 2026

Isolation of Human Neutrophils from Whole Blood and Buffy Coats

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Published on: September 17, 2021

Three-Dimensional Cell Culture Models to Investigate the Epithelial Barrier in Eosinophilic Esophagitis

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Published on: May 10, 2024

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation

Published on: May 30, 2020

Area of Science:

Immunology
Hematology
Cell Biology

Background:

Eosinophils play crucial roles in immune responses, particularly in allergic reactions and defense against parasites.
Accurate isolation of eosinophils is essential for studying their function and for developing targeted therapies.

Purpose of the Study:

To describe a reliable and efficient protocol for the isolation of highly purified eosinophils from peripheral blood.
To provide supporting methods for assessing eosinophil purity and differentiating them from other cell types.

Main Methods:

Utilizes antibody-based magnetic negative selection for isolating eosinophils from peripheral blood.
Involves sequential fractionation, enriching for CD16+ granulocytes first, followed by eosinophil isolation.
Includes staining methods for purity assessment and differentiation from other leukocytes.

Main Results:

The described protocol enables the isolation of highly purified eosinophils.
The technique is characterized by its ease of use, speed, and high reproducibility.
Staining methods effectively evaluate eosinophil purity and distinguish them from other leukocyte populations.

Conclusions:

This antibody-based magnetic negative selection protocol offers an efficient and reproducible method for obtaining pure eosinophils.
The protocol is valuable for immunological and hematological research requiring pure eosinophil populations.
The supporting staining methods enhance the reliability of eosinophil isolation studies.