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Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
Published on: July 23, 2015
Neurohistochemistry in molluscan species: focus on extracellular matrix
Nitin A Kamble1, Sunil R Londhe
1Department of Zoology, Shivaji University, Kolhapur 416 004, India. drknitinkumar@yahoo.in
Abstract:
Carbohydrate polymers with acid-base properties of extracellular matrix (ECM) have a critical impact on guidance, migration and synaptic transmission. The present study showed acid-base properties of ECM stained with histochemical techniques. The central nervous system (CNS) of mature terrestrial slug Semperula maculata and freshwater bivalve Lamellidens corrianus was selected to visualize sulphated and carboxylic strong acid groups. Protein and carbohydrate molecules were found scattered in three different regions, viz. periganglionic sheath, along with the basement lamina, interperikaryal space including the perineuronal net, and neuropil, in both the molluscan species. Sulphated proteoglycans were abundant in periganglionic sheath of S. maculata and also occurred in the neuropil of L. corrianus. The interperikaryal space contained carboxyl groups along with hyaluronic acid, and neuropil contained abundant glycogen moiety in both species. Comparing the SDS-PAGE of the neural tissue extracts revealed protein molecules that were found in the same fraction. The ECM of neuronal cells from S. maculata and L. corrianus presented different kinds of glycoproteins and proteoglycans, which showed its morphological as well as habitual differentiation.
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