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Updated: May 19, 2026
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Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
Published on: November 11, 2014
Eeny meeny miny moe, catch a transcript by the toe, or how to enumerate eukaryotic transcripts
Terence R Strick1, Nouria Hernandez
1Institut Jacques Monod, CNRS UMR, University of Paris-Diderot, France. strick.terence@ijm.univ-paris-diderot.fr
Genes & Development
|August 3, 2012
Summary
Researchers quantified transcription factor assembly during RNA polymerase II transcription. This study links individual transcription events to the precise timing of transcription factor binding on DNA.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Transcription is a fundamental biological process involving RNA polymerase II.
- Understanding the dynamics of transcription factor assembly is crucial for gene regulation.
Purpose of the Study:
- To investigate the relationship between individual RNA polymerase II transcription events and transcription factor assembly.
- To determine the temporal correlation between transcriptional activity and protein binding dynamics.
Main Methods:
- Utilized single-molecule fluorescence to count RNA transcripts retained on template DNA.
- Developed a method to measure transcription events at the single-molecule level.
Main Results:
- Established a quantitative link between individual RNA polymerase II transcription events and transcription factor binding.
- Demonstrated the ability to measure transcription factor assembly in real-time relative to transcription initiation.
Conclusions:
- Provides a novel method for studying transcription dynamics at high resolution.
- Offers insights into the precise mechanisms of transcription regulation and gene expression control.
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00:58Eukaryotic RNA Polymerases
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All three eukaryotic RNAPs require specific transcription factors, of which the...
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00:58Eukaryotic RNA Polymerases
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
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The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
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