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Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

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Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
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Atmospheric scanning electron microscope for correlative microscopy.

Ian E G Morrison1, Clare L Dennison, Hidetoshi Nishiyama

  • 1Technology Facility, Biology Department, University of York, York YO10 5DD, UK.

Methods in Cell Biology
|August 4, 2012
PubMed
Summary
This summary is machine-generated.

The JEOL ClairScope integrates scanning electron microscopy (SEM) and optical microscopy for correlative imaging of wet samples. This novel microscope enables simultaneous observation and manipulation of biological specimens under ambient conditions.

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Area of Science:

  • Correlative microscopy
  • Electron microscopy
  • Optical microscopy
  • Nanotechnology

Background:

  • Traditional microscopy methods often struggle with preserving sample integrity and performing correlative imaging.
  • Observing wet biological samples under electron microscopy typically requires harsh fixation and vacuum conditions, limiting in-situ analysis.

Purpose of the Study:

  • To introduce the JEOL ClairScope, the first integrated scanning electron and optical microscope designed for correlative imaging.
  • To demonstrate the capability of imaging wet samples in ambient conditions, allowing for biochemical modifications.

Main Methods:

  • Utilizes an inverted scanning electron microscope (SEM) column with a silicon nitride window for sample observation in a culture dish.
  • Incorporates an inverted optical microscope for reflected light and epifluorescence imaging of the same sample.
  • Employs nanophosphor particles to enhance correlation between optical and electron images via cathodoluminescence.

Main Results:

  • Achieved correlative imaging of wet samples under ambient conditions, preserving biological integrity.
  • Enabled sequential staining and biochemical modifications directly on the sample within the microscope.
  • Demonstrated effective correlation between optical and SEM images using nanophosphor particles for improved spatial registration.

Conclusions:

  • The JEOL ClairScope offers a groundbreaking platform for correlative microscopy, bridging the gap between optical and electron imaging.
  • This technology facilitates advanced in-situ analysis of biological samples, opening new avenues for research in cell biology and materials science.