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Updated: May 19, 2026
Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model
Published on: February 17, 2023
Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in
Bruno B Benatti1, Jozafá C Campos-Júnior, Vilmar J Silva-Filho
1Laboratory of Biopathology and Molecular Biology, University of Uberaba, Uberaba, MG, Brazil. brunobbenatti@yahoo.com.br
Objectives:
The Mikania laevigata extract (MLE) (popularly known in Brazil as "guaco") possesses anti-inflammatory properties. In the present study we tested the effects of MLE in a periodontitis experimental model in rats. We also investigated possible mechanisms underlying such effects.
Material And Methods:
Periodontal disease was induced by a ligature placed around the mandibular first molars of each animal. Male Wistar rats were divided into 4 groups: non-ligated animals treated with vehicle; non-ligated animals treated with MLE (10 mg/kg, daily); ligature-induced animals treated with vehicle and ligature-induced animals treated with MLE (10 mg/kg, daily). Thirty days after the induction of periodontal disease, the animals were euthanized and mandibles and gingival tissues removed for further analysis.
Results:
Morphometric analysis of alveolar bone loss demonstrated that MLE-treated animals presented a decreased alveolar bone loss and a lower expression of the activator of nuclear factor-κB ligand (RANKL) measured by immunohistochemistry. Moreover, gingival tissues from the MLE-treated group showed decreased neutrophil migration myeloperoxidase (MPO) assay.
Conclusions:
These results indicate that MLE may be useful to control bone resorption during progression of experimental periodontitis in rats.
