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Updated: May 19, 2026

13:03
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
Increasing the efficiency of digitization workflows for herbarium specimens.
Melissa Tulig1, Nicole Tarnowsky, Michael Bevans
1William and Lynda Steere Herbarium, The New York Botanical Garden, Bronx, New York, USA.
Zookeys
|August 4, 2012
Summary
Digitizing 7.3 million plant specimens is slow, taking 130,000 hours for 1.3 million. New methods and optical character recognition (OCR) aim to speed up the process for critical biodiversity data.
Area of Science:
- Botany
- Biodiversity Informatics
- Digital Curation
Background:
- The New York Botanical Garden Herbarium houses an estimated 7.3 million plant specimens, representing a significant botanical resource.
- Digitization efforts have been ongoing for 17 years, focusing on fundable subsets due to collection size.
- Current digitization rates are insufficient to complete the collection within realistic time and budget constraints.
Purpose of the Study:
- To evaluate and implement new strategies for accelerating the digitization of large herbarium collections.
- To explore cost-effective and time-efficient methods for data capture and specimen imaging.
- To prepare for larger-scale digitization projects through programs like the National Science Foundation's Advancing Digitization of Biological Collections.
Main Methods:
- Selective digitization of fundable subsets of specimens over successive grant cycles.
- Testing new protocols including data collaboration, field book digitization, and partial data entry/imaging.
- Utilizing optical character recognition (OCR) technology for specimen image data extraction.
Main Results:
- To date, 1.3 million specimens have been databased, requiring approximately 130,000 staff hours.
- The current rate averages 10 specimens per hour, projecting a need for an additional 600,000 hours to complete the collection.
- New methods are being tested to significantly increase digitization efficiency and reduce costs.
Conclusions:
- The traditional rate of herbarium specimen digitization is unsustainable given current biodiversity and economic challenges.
- Innovative approaches, including OCR and collaborative data efforts, are crucial for efficient and timely digitization.
- Securing funding and leveraging new programs are essential for advancing the digitization of biological collections.

