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Related Experiment Video

Updated: May 19, 2026

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening
08:14

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening

Published on: October 26, 2017

Comparing the MicroRNA spectrum between serum and plasma.

Kai Wang1, Yue Yuan, Ji-Hoon Cho

  • 1Institute for Systems Biology, Seattle, Washington, United States of America. kwang@systemsbiology.org

Plos One
|August 4, 2012
PubMed
Summary

Circulating microRNAs (miRNAs) in blood are potential biomarkers. This study found that serum has higher miRNA levels than plasma, influenced by sample type and coagulation, impacting biomarker reliability.

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Biomarker Discovery

Background:

  • MicroRNAs (miRNAs) are small, non-coding RNAs regulating gene expression.
  • Circulating miRNAs in blood show potential as biomarkers for diseases like cancer.
  • Understanding factors affecting miRNA detection is crucial for reliable biomarker use.

Purpose of the Study:

  • To investigate the impact of different blood sample types (serum vs. plasma) on circulating miRNA levels.
  • To explore how sample processing and measurement platforms influence miRNA detection.

Main Methods:

  • Comparison of miRNA concentrations in paired serum and plasma samples from the same individuals.
  • Analysis of potential associations between miRNA levels and factors like platelet content and coagulation.

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Isolation of Small Noncoding RNAs from Human Serum
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Isolation of Small Noncoding RNAs from Human Serum

Published on: June 19, 2014

Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR
10:23

Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR

Published on: February 12, 2018

Related Experiment Videos

Last Updated: May 19, 2026

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening
08:14

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening

Published on: October 26, 2017

Isolation of Small Noncoding RNAs from Human Serum
06:44

Isolation of Small Noncoding RNAs from Human Serum

Published on: June 19, 2014

Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR
10:23

Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR

Published on: February 12, 2018

  • Evaluation of miRNA measurements across different experimental platforms.

    • Main Results:

    • Serum samples exhibited significantly higher concentrations of circulating miRNAs than corresponding plasma samples.
    • Observed differences in miRNA levels between serum and plasma were partly associated with platelet-derived miRNAs, suggesting a role for coagulation.
    • Variations in miRNA measurements were also noted depending on the detection platform used.

    Conclusions:

    • Sample type (serum vs. plasma) and processing (coagulation) significantly affect circulating miRNA concentrations.
    • Measurement platform can introduce variability, impacting data comparability.
    • Standardization of sample handling and measurement techniques is essential for accurate and reproducible miRNA biomarker studies.