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Related Concept Videos

Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Related Experiment Video

Updated: May 19, 2026

Using the BLT Humanized Mouse as a Stem Cell based Gene Therapy Tumor Model
06:59

Using the BLT Humanized Mouse as a Stem Cell based Gene Therapy Tumor Model

Published on: December 18, 2012

Humanized c-Myc mouse.

Frank M Lehmann1, Samantha Feicht, Florian Helm

  • 1Institute of Clinical Molecular Biology and Tumor Genetics, Helmholtz Center Munich, Munich, Germany.

Plos One
|August 4, 2012
PubMed
Summary

Researchers developed a new mouse model expressing human c-MYC, which can replace the mouse version. This hc-Myc mouse strain is crucial for studying MYC-targeted cancer therapies and their side effects.

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Testing Cancer Immunotherapeutics in a Humanized Mouse Model Bearing Human Tumors
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Testing Cancer Immunotherapeutics in a Humanized Mouse Model Bearing Human Tumors

Published on: December 16, 2022

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Last Updated: May 19, 2026

Using the BLT Humanized Mouse as a Stem Cell based Gene Therapy Tumor Model
06:59

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Published on: December 18, 2012

Testing Cancer Immunotherapeutics in a Humanized Mouse Model Bearing Human Tumors
15:24

Testing Cancer Immunotherapeutics in a Humanized Mouse Model Bearing Human Tumors

Published on: December 16, 2022

Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • Tumors often rely on specific oncogenes, a concept known as oncogene addiction.
  • Targeting oncogene products offers therapeutic strategies but faces challenges due to potential toxicity in normal cells.
  • A therapeutic window is needed to balance anti-tumor effects with adverse effects on normal tissues.

Purpose of the Study:

  • To develop a mouse model for studying therapies targeting the human c-MYC proto-oncogene.
  • To create a system that allows expression of human c-MYC in both tumor and normal cells.
  • To investigate the therapeutic window for c-MYC-targeted treatments.

Main Methods:

  • Generated C57BL/6 embryonic stem cells engineered with a humanized c-Myc gene.
  • Established a mouse strain (hc-Myc) where human c-MYC replaces the endogenous murine c-Myc.
  • Integrated the humanized c-Myc gene into the murine c-Myc locus.

Main Results:

  • The hc-Myc mouse strain successfully expresses human c-MYC in place of the murine ortholog.
  • Homozygous hc-Myc mice exhibit a normal phenotype, indicating functional replacement by human c-MYC.
  • Human c-MYC can fully substitute for murine c-MYC function in vivo.

Conclusions:

  • The hc-Myc mouse strain serves as a valuable model for examining adverse effects of human c-MYC-targeting therapies.
  • Cross-breeding hc-Myc mice with tumor-prone models will enable simultaneous evaluation of therapeutic efficacy and toxicity.
  • This model facilitates comprehensive study of MYC-specific therapies in a clinically relevant context.