Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In Remembrance of Professor Zelig Eshhar: <i>A Life Committed to CAR-T</i>.

Human gene therapy·2026
Same author

Opposing Association of Lung Neutrophils and PD-L1<sup>+</sup> Monocytes in Age-Related Severity of SARS-CoV-2 Infection.

Aging cell·2026
Same author

Affinity-matured B cell responses neutralizing type-I interferons underlie severe viral infections.

Cell·2026
Same author

Systemic immune dysregulation and neutrophil activation define prognostic inflammatory signatures in drug-resistant epilepsy.

JCI insight·2026
Same author

In Remembrance of Professor Zelig Eshhar: <i>A Life Committed to CAR-T</i>.

Human gene therapy·2026
Same author

Defective RNA Polymerase III sensing of mitochondrial DNA in pulmonary epithelial cells impairs type I IFN immunity to SARS-CoV-2.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: May 19, 2026

Rapid Development of Cell State Identification Circuits with Poly-Transfection
09:21

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

Evaluating cellular polyfunctionality with a novel polyfunctionality index.

Martin Larsen1, Delphine Sauce, Laurent Arnaud

  • 1Institut National de la Santé et de la Recherche Médicale UMR-S 945, Paris, France. Martin.Larsen@upmc.fr

Plos One
|August 4, 2012
PubMed
Summary

Researchers developed a novel polyfunctionality index to better analyze T cell responses. This new index numerically evaluates T cell polyfunctionality, enabling advanced statistical analysis and more precise comparisons in immunological studies.

More Related Videos

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

Related Experiment Videos

Last Updated: May 19, 2026

Rapid Development of Cell State Identification Circuits with Poly-Transfection
09:21

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

Area of Science:

  • Immunology
  • Cellular Biology
  • Biotechnology

Background:

  • T cell responses are increasingly measured using multidimensional analyses, like multiparametric flow cytometry.
  • Analyzing complex datasets of T cell functional molecules (cytokines, chemokines) at the single-cell level is challenging.
  • Current methods using pie charts lack detail on polyfunctionality variation and statistical robustness.

Purpose of the Study:

  • To introduce a novel polyfunctionality index for quantitative T cell response analysis.
  • To enable more sophisticated statistical analysis of T cell polyfunctionality.
  • To establish polyfunctionality as a robust endpoint measure in immunological research.

Main Methods:

  • Development of a novel numerical index to quantify T cell polyfunctionality.
  • Application of the index for comparative and correlative statistical tests (parametric and non-parametric).
  • Integration of advanced statistical approaches like cluster analysis.

Main Results:

  • The proposed index numerically evaluates both the degree and variation of T cell polyfunctionality.
  • It facilitates advanced statistical analyses, including cluster analysis.
  • The index allows for more robust statistical comparisons of T cell responses.

Conclusions:

  • The novel polyfunctionality index offers a significant advancement in analyzing complex T cell functional data.
  • This index overcomes limitations of traditional methods like pie charts.
  • It is expected to become a valuable tool for future immunological studies evaluating T cell responsiveness.