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Cancer Stem Cells and Tumor Maintenance

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...

Cancer Stem Cells and Tumor Maintenance

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells

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Stem Cell Therapy for Tissue Regeneration

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Related Experiment Video

Updated: May 19, 2026

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

Published on: March 1, 2020

Ovarian cancer stem cells.

A G Zeimet¹, D Reimer, S Sopper

¹Department of Obstetrics and Gynecology, Innsbruck Medical University, Innsbruck, Austria. alain.zeimet@i-med.ac.at

|August 7, 2012

Summary

This summary is machine-generated.

Identifying ovarian cancer stem cells is challenging due to a lack of universal markers. This study explored side-population (SP) and ALDH1A1+ cells, finding they represent distinct, overlapping fractions crucial for tumor growth and drug resistance.

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Area of Science:

Oncology
Cancer Stem Cell Biology
Molecular Biology

Background:

Advanced ovarian cancer presents as numerous peritoneal nodules, making it a suitable model for cancer stem cell (CSC) research.
Isolating CSCs, characterized by anoikis-resistance and self-renewal, remains a significant challenge due to the absence of a universal marker.
Cancer stem cells possess efflux systems that can be exploited for isolation via dye exclusion (side-population, SP) or cell surface markers.

Purpose of the Study:

To establish and characterize a proof-of-principle model for ovarian cancer stem cells using the A2780V cell line.
To investigate the distinct properties of side-population (SP) and ALDH1A1+ cells in ovarian cancer.
To explore the role of these cell populations in chemoresistance and their relationship with specific gene expression.

Main Methods:

Established a stable side-population (SP) and ALDH1A1+ cell population in the A2780V ovarian cancer cell line.
Utilized flow cytometry for cell isolation and analysis of surface markers (CD44, CD117).
Assessed spheroid formation on mesothelial cells and platinum drug resistance, alongside ERCC1 gene expression analysis.

Main Results:

The SP and ALDH1A1+ cell fractions in A2780V cells were found to be distinct populations.
A2780V SP cells exhibited spheroid formation and partial resistance to platinum, independent of CD44/CD117 expression.
ERCC1 gene was over-expressed in sphere-cultured cells (stem-like) compared to adherent cells (differentiated), suggesting a role in chemoresistance under stem cell conditions.

Conclusions:

Hierarchical growth and differentiation are conserved even in multi-passaged ovarian cancer cell lines.
The key cancer stem cell population may comprise small, overlapping fractions identified by various markers.
Understanding these distinct cell populations is crucial for developing targeted ovarian cancer therapies.