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Inoculation Strategies to Infect Plant Roots with Soil-Borne Microorganisms

Inoculation Strategies to Infect Plant Roots with Soil-Borne Microorganisms

Published on: March 1, 2022

Rhizobacteria Bacillus subtilis restricts foliar pathogen entry through stomata.

Amutha Sampath Kumar¹, Venkatachalam Lakshmanan, Jeffrey L Caplan

¹Department of Plant and Soil Sciences, University of Delaware, Newark, DE 19716, USA.

The Plant Journal : for Cell and Molecular Biology

|August 7, 2012

Summary

Rhizobacteria Bacillus subtilis FB17 (FB17) colonization of plant roots triggers abscisic acid and salicylic acid pathways. This closes stomata, restricting the entry of the bacterial pathogen Pseudomonas syringae pathovar tomato DC3000 (PstDC3000).

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Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates

Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates

Published on: July 2, 2018

Area of Science:

Plant pathology
Microbial ecology
Plant immunity

Background:

Plants face multitrophic interactions and possess defense mechanisms like physical barriers and stomatal closure.
Bacterial pathogens such as Pseudomonas syringae pathovar tomato DC3000 (PstDC3000) primarily invade plants through stomata.
Plant innate immunity includes transient stomatal closure to delay disease progression.

Purpose of the Study:

To investigate if root colonization by Bacillus subtilis FB17 (FB17) can restrict stomatal-mediated entry of PstDC3000 in Arabidopsis thaliana.
To elucidate the signaling pathways involved in FB17-induced stomatal closure.

Main Methods:

Arabidopsis thaliana were inoculated with Bacillus subtilis FB17 (FB17) on their roots.
The effect of FB17 root colonization on PstDC3000 entry was assessed.
Abscisic acid (ABA) and salicylic acid (SA) signaling pathways were analyzed for their role in FB17-induced stomatal closure.

Main Results:

Root colonization by FB17 was found to restrict stomatal-mediated entry of PstDC3000.
FB17 root binding activated abscisic acid (ABA) and salicylic acid (SA) signaling pathways.
These activated pathways led to the closure of light-adapted stomata.

Conclusions:

Rhizospheric processes, specifically root colonization by FB17, play a crucial role in plant defense against foliar bacterial pathogens.
The activation of ABA and SA pathways by FB17 is a key mechanism for inducing stomatal closure and limiting pathogen entry.
Environmental conditions and rhizospheric interactions are integral components of the plant innate immune system.