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Related Experiment Video

Updated: May 19, 2026

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
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Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

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Development of a porcine (Sus scofa) embryo-specific microarray: array annotation and validation.

Stephen Tsoi1, Chi Zhou, Jason R Grant

  • 1Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada. stsoi1@ualberta.ca

BMC Genomics
|August 7, 2012
PubMed
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Researchers developed a novel porcine-embryo-specific microarray (EMPV1) using deep sequencing. This tool will advance understanding of factors influencing pig embryo and offspring viability.

Area of Science:

  • Reproductive Biology
  • Genomics
  • Bioinformatics

Background:

  • Domestic pigs are vital livestock with significant interest in embryo and offspring development.
  • Limited understanding of molecular mechanisms in early embryonic development hinders progress.
  • Next-generation sequencing and microarray technologies offer powerful tools for studying embryonic molecular pathways.

Purpose of the Study:

  • To develop a porcine-embryo-specific microarray platform.
  • To facilitate research into factors affecting porcine embryo viability and offspring development.

Main Methods:

  • Expressed sequence tag (EST) analysis using Roche/454 next-generation sequencing of cDNAs from porcine preimplantation embryos.
  • Development of the EMbryogene Porcine Version 1 (EMPV1) microarray with 43,795 probes.

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Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR
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Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
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Published on: January 30, 2017

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  • Quality control and re-annotation of probe sequences.

    • Main Results:

    • Generated over one million high-quality EST sequences.
    • The EMPV1 microarray represents an estimated 20,000 unique genes, including protein-coding genes, non-coding RNAs, and splice variants.
    • High reproducibility (0.97 array-to-array correlation) confirmed the microarray's quality.

    Conclusions:

    • Next-generation sequencing enabled the creation of a comprehensive EST dataset for microarray development.
    • The EMPV1 microarray is a high-quality, reproducible platform for studying porcine embryo development.
    • This platform will serve as a foundation for future research on factors influencing porcine embryo and offspring viability.