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Published on: September 9, 2012
Factor X-activating procoagulant in normal and malignant breast tissue
K el-Baruni1, I Taylor, S Roath
1University Department of Haematology, Southampton General Hospital, U.K.
Hematological Oncology
|November 1, 1990
Summary
Factor X-activating activity (FXAA) was found in both normal and malignant breast tissue. This serine protease activity, similar in both tissue types, is linked to a tissue factor-factor VII complex.
Area of Science:
- Biochemistry
- Oncology
- Hematology
Background:
- Factor X-activating activity (FXAA) is crucial in coagulation.
- Understanding FXAA in breast tissue is important for diagnostics and therapeutics.
Purpose of the Study:
- To determine the presence and characteristics of FXAA in normal and malignant breast tissue.
- To investigate the relationship between FXAA and tissue hemoglobin levels.
Main Methods:
- Chromogenic assay used to measure FXAA in 38 breast tissue samples.
- Inhibition studies conducted using various agents (e.g., DFP, PMSF, phospholipase C).
Main Results:
- FXAA detected in all tested breast tissues, regardless of pathology.
- Similar FXAA levels observed in normal and malignant tissues.
- FXAA correlated with hemoglobin in normal breast tissue (p < 0.02), but not in tumors.
Conclusions:
- FXAA in breast tissue is a serine protease, likely a tissue factor-factor VII complex.
- FXAA is present in both normal and malignant breast tissue.
- Observed FXAA in normal tissue may be an artifact of sample preparation.
