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Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
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Published on: December 31, 2017

Microbiological characterization in children with aggressive periodontitis.

L M Shaddox1, H Huang, T Lin

  • 1Department of Periodontology, College of Dentistry, University of Florida, Gainesville, FL, USA. lshaddox@dental.ufl.edu

Journal of Dental Research
|August 7, 2012
PubMed
Summary

Localized Aggressive Periodontitis (LAP) in African-American children is strongly associated with Aggregatibacter actinomycetemcomitans (Aa) and Filifactor alocis (Fa) in diseased sites. Healthy sites harbor different bacterial communities, highlighting site-specific microbial profiles in LAP.

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In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
11:15

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Published on: May 21, 2015

Area of Science:

  • Microbiology
  • Periodontology
  • Pediatric Dentistry

Background:

  • Localized Aggressive Periodontitis (LAP) is a severe form of gum disease.
  • Understanding the subgingival microbiota in affected children is crucial for diagnosis and treatment.
  • African-American children represent a specific demographic for this study.

Purpose of the Study:

  • To characterize the subgingival bacterial community in African-American children with LAP.
  • To identify specific bacterial species associated with diseased and healthy sites in LAP patients.
  • To compare the microbiota of LAP patients with healthy controls.

Main Methods:

  • Subgingival plaque samples collected from diseased and healthy sites in LAP patients and healthy controls.
  • Analysis using 16S rRNA-based microarrays for microbial identification and quantification.
  • Statistical analysis to determine species prevalence and abundance differences.

Main Results:

  • Aggregatibacter actinomycetemcomitans (Aa) was significantly more prevalent and abundant in diseased sites of LAP patients.
  • Filifactor alocis (Fa) also showed increased prevalence in diseased sites.
  • Healthy sites were characterized by species like Selenomonas spp., Veillonella spp., and Streptococcus spp.

Conclusions:

  • Aggregatibacter actinomycetemcomitans (Aa) is a key bacterial species strongly associated with diseased sites in pediatric LAP.
  • The subgingival microbiota composition differs significantly between diseased and healthy sites in LAP.
  • Specific bacterial profiles are linked to healthy individuals, distinct from those in LAP.