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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
PCR01:32

PCR

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PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

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Related Experiment Video

Updated: May 19, 2026

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
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Published on: March 30, 2015

qRT-PCR: a method and its difficulties

Franziska Bollmann, Ingrid Casper, Jenny Henke

    Naunyn-Schmiedeberg'S Archives of Pharmacology
    |August 7, 2012
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    No abstract available in PubMed .

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