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Updated: May 19, 2026

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
Published on: March 19, 2018
Comparison of the multiple-sample means with composite sample results for fecal indicator bacteria by quantitative
Reagan R Converse1, Larry J Wymer, Alfred P Dufour
1Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA. converse.reagan@epa.gov
Abstract:
Few studies have addressed the efficacy of composite sampling for measuring indicator bacteria by quantitative PCR (qPCR). We compared results from composited samples with multiple-sample means for culture- and qPCR-based water quality monitoring. Results from composited samples for both methods were similarly correlated to multiple-sample means and predicted criteria exceedances equally.
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